Composite

Part:BBa_K4206003:Design

Designed by: Kazuma Tsurumaki   Group: iGEM22_Qdai   (2022-10-04)


cellulases with PhoA signal sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 4670
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 4303
    Illegal NgoMIV site found at 4676
    Illegal NgoMIV site found at 5178
    Illegal AgeI site found at 331
    Illegal AgeI site found at 1795
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4723
    Illegal SapI.rc site found at 4806


Design Notes

These parts optimized to remove illegal restriction sites.


Source

Sequences taken from the following part pages. BBa_K3394000,BBa_K1131002,BBa_K2449012,BBa_K1499501

References